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Following clearance via centrifugation, protein concentration of lysates was determined using Pierce BCA Protein Assay (23225, Fisher Cientific) and 30g of lysate was prepared using 4x LDS sample buffer (Thermo Fisher) and 10% 2-mercaptoethanol and run on NuPAGE 4-15% bis-tris gels (Thermo Fisher)

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In another acute toxicity study, no toxicity or mortality was observed, even at doses up to 5,000 mg/kg (637, 705)

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